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KMID : 0615720120290010067
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Jouranl of Korean Acupuncture and Moxibustion society
2012 Volume.29 No. 1 p.67 ~ p.74
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Effects of Hot Aqueous and Ethanol Extract from Lonicera japonica Flos on NO and PGE2 in Macrophage
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Yoon Kyung-Jin
Lee Eun-Yong
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Abstract
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Objectives: The objective of this study is to study the effects of hot aqueous extract and ethanol extract from Lonicera japonica Flos on nitric oxide(NO) and prostaglandin E2(PGE2) production in macrophage.
Methods: Lonicera japonica Flos was extracted in two ways. One was extracted with distilled water(2L) for 4 h and the other one was extracted with 70% ethanol (2L) for 4h. The RAW 264.7 macrophage was subclutured. In order to evaluate cytotoxicity, MTT assay was performed. The concentrations of NO were measured by Griess assay. The concentrations of PGE2 were measured by enzyme immunoassay.
Results: 25, 125 ㎍/㎖ hot aqueous extract from Lonicera japonica Flos inhibited NO production in LPS-stimulated RAW 264.7 macrophages significantly. 25, 125, 625 ㎍/㎖ ethanol extract from Lonicera japonica Flos inhibited NO production in LPS-stimulated RAW 264.7 macrophages significantly. 150, 200 ㎍/㎖ hot aqueous extract and ethanol extract from Lonicera japonica Flos inhibited PGE2 production in LPS-stimulated RAW 264.7 macrophages significantly.
Conclusions: This study suggests that hot aqueous extract and ethanol extract from Lonicera japonica Flos suppress NO and PGE2 production. So hot aqueous extract and ethanol extract from Lonicera japonica Flos may have an anti-inflammation effect.
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KEYWORD
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Lonicera japonica Flos, NO, PGE2, macrophage, cytotoxicity, anti-inflammation, hot aqueous extract, ethanol extract
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